Background: In resource-limited settings, the frequency of exposure of Zygomycetes from an environmental source, the influence of geographical location, and the minimum infective doses remain a public health challenge. Objective: To outline the environmental distribution of medically important Zygomycetes species in the Bushenyi district of Uganda. Materials and Methods: Standard Mycological methods were used in the isolation and phenotypic identification of zygomycetes in 120 soil samples randomly collected from suspected zygomycetes habitable sites in different representative locations in the Bushenyi district of Uganda. Results. We found a 47% prevalence of zygomycetes species from the samples analyzed. Out of the 56 isolates identified using colonial morphology, slide cultures were performed to identify them further. 20 (35.7%) were Rhizopus, 16 (28.6%) Rhizomucor, 15 (26.8%) Mucor, 3 (5.4%) Absidia, 1 (1.8%) Apophysomyces and 1(1.8%) Cunninghamella. Conclusion: The soil samples analyzed contained a wide array of zygomycetes species comparable to the findings of other researches in global and African literature. The medical and public health importance of the isolated zygom
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