Biomedical applications of green synthesized silver nanoparticles from Prosopis juliflora

The present study explores the phytochemical properties and green synthesis of silver nanoparticles using the ethanol extract of Prosopis juliflora, a medicinal plant known for its bioactive constituents. Plant samples were aseptically collected from the Davangere district, Karnataka, and processed through washing, shade drying, and grinding. The crude extract was obtained using cold maceration with ethanol, followed by filtration and drying. Qualitative phytochemical screening confirmed the presence of secondary metabolites including phenols, tannins, saponins, and glycosides. UV-Visible spectrophotometry revealed an absorption peak at 434 nm, indicating the presence of bioactive compounds. FTIR spectroscopy identified functional groups involved in the biochemical profile of the extract. GC-MS analysis detected 35 secondary metabolites in the extract, with major constituents such as Ethane, 1,1-diethoxy-, Propane, 2,2-diethoxy-, and 4-O-Methylmannose. The green synthesis of silver nanoparticles was achieved by adding 1 mM AgNO₃ to the ethanol extract, confirmed by a color change from light brown to dark brown. The synthesized Pj-AgNPs were characterized by UV-Vis spectroscopy, sho