Characterization of <i>Vibrio</i> spp. in environmental water samples collected from flood prone areas of Bangladesh and their antibiotic resistance profile

<abstract> <p>Last cholera epidemic has been recorded in Bangladesh between 1992–1993, while few sporadic localized outbreaks have been reported as recent as 2005. Serotype O1 of <italic>Vibrio cholera</italic> is considered as the principal causative agent which transmits through contaminated drinking water resulting that epidemic. Therefore, the objective of this research was to isolate <italic>V. cholera</italic> in 3 different water sources; River, pond and tube-well, in 5 different locations of Gazipur, Bangladesh, and to analyze their antibiogram study. A total of 45 water samples were randomly collected for the isolation and identification of <italic>Vibrio</italic> spp. Samples are then serially diluted in alkaline peptone water and streak on Thiosulfate Citrate Bile Salt Sucrose-TCBS agar for quantification of <italic>V</italic>. spp. For <italic>V. cholera</italic> isolation water samples were first enriched in nutrient broth at 37 °C for 16 hours followed by cultivation in selective media; TCBS agar at 37 °C for 24 hours. Yellow colonies on TCBS agar were screed as <italic>V. cholera</italic