The priority task of modern biotechnology is development of the rational technologies for the microbial synthesis of practically important products. Among these products, a significant place belongs to surfactants (biosurfactants), which are widely used in many sectors of the economy. The most problematic stage of the biosurfactants production is isolation from the post fermentative cultural liquid of bacteria-producers. Improving the efficiency of the biosurfactants production is highly dependent on rational approaches to the target products isolation. In this regard, there is an increasing need for rational, scientifically substantiated methods for their isolation and purification. Therefore, the aim of the presented work was to determine the optimal extractants for the isolation of rhamnolipid surfactants – metabolites of bacteria of Pseudomonas sp. PS-17 strain. For this purpose, the extraction process of rhamnolipids from the post fermentative cultural liquid supernatant has been investigated. The optimal extractants were selected among 13 organic solvents of different nature. Processing of the obtained experimental data by the method of multi-parameter equations of linearity
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