Purpose of the study: The purpose of this study is to investigate the relationship between self-regulated learning (SRL) and students’ critical thinking and scientific communication skills in Biology learning. Specifically, it aims to describe students’ SRL, critical thinking, and communication levels, and analyze how SRL influences both skills in a multicultural bilingual context. Methodology: This study employed an explanatory sequential mixed method. The quantitative phase involved 60 grade XI students at Sekolah Indonesia Davao using total sampling, with data collected through SRL questionnaires, critical thinking tests, and scientific communication rubrics. The qualitative phase involved three students and two teachers via semi-structured interviews. Data were analyzed using descriptive statistics, regression, and Miles–Huberman’s model. Main Findings: The findings showed students’ SRL was moderate to high (M = 3.70), strongest in goal setting and weakest in environmental control. Critical thinking (M = 3.53) and scientific communication (M = 3.51) were moderate, with strengths in problem clarification and claim–evidence–reasoning, respectively. Regression analysis revealed
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