COLLECTION OF THE STRAINS TESCHOVIRUS A, SAPELOVIRUS A, ENTEROVIRUS G OF THE INSTITUTE OF AGRICULTURAL MICROBIOLOGY AND AGROINDUSTRIAL MANUFACTURE OF THE NAAS

Objective. Arrangement of the collection of porcine enteroviruses (PEV) strains isolated on the territory of Ukraine in accordance with the requirements of the International Committee on Virus Taxonomy and supplementing it with new strains Teschovirus A (TV-A), Sapelovirus A (SV-A), Enterovirus G (EV-G). Methods. Virological, serological, molecular genetic, instrumental and statistical. Isolation, cultivation of viruses and determination of their biological activity were performed in passaged culture of porcine embryonic kidney cells (СНЕВ). The viral titre was calculated by the method of Reed and Muench. The typical affiliation of viruses was determined in the virus neutralization reaction in СНЕВ cell culture. Species affiliation was determined by reverse transcription polymerase chain reaction (RT PCR) using species-specific primers for TV-A, SV-A and EV-G, developed by us. Electron microscopy of viruses was performed on a transmission electron microscope by negative contrast enhancement method. Statistical processing was performed in Microsoft Office Excel and StatSoft STATISTICA 12. Results. As a result of the epizootic survey during 2002– 2019, 1,216 samples for virological t