Background Beekeepers are at increased risk for anaphylaxis due to frequent honeybee stings. This study developed an educational program for anaphylaxis prevention and an assessment tool to evaluate the knowledge and attitudes of Slovenian beekeepers. Methods The educational program was developed using the Delphi method with 13 healthcare professionals (9 allergists and 4 registered nurses specialized in allergology). The Beekeepers Anaphylaxis Awareness and Learning Questionnaire (BAALQ) consisted of two distinct components, developed by 12 experts. Content validity for the Recognition and Anaphylaxis Action Scale (RAAS) was confirmed by 59 healthcare professionals. Criterion and construct validity were assessed with 143 beekeepers without a history of anaphylaxis, using a nominal scale for measuring knowledge and a 5-point Likert scale for evaluating Socio-Cultural Learning Attitudes Scale (SCLAS). Knowledge differences among beekeepers before and after the educational intervention were evaluated using the Wilcoxon test. Cronbach’s Alpha coefficient was used to a
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