Production of antimicrobial compounds in <i>Lactobacillus brevis</i> cells

As a result of the study, primary data were obtained on the kinetics of growth and production of antimicrobial compounds by the bacteria Lactobacillus brevis 2kGv22-17. Under experimental conditions, the antibacterial effect of a suspension of lactobacilli, protoplasts and intracellular antibacterial activity in cell lysates were studied. Growth kinetics were assessed under static conditions using a personal bioreactor RTS-1C. The antagonistic activity of lactobacilli was studied in a series of experiments using Cytophaga psychrophila IM-87, Flexibacter columnaris IM-216 and Pseudomonas fluorescens IM-43 as indicator test cultures. The ability of the studied lactobacilli to produce antimicrobial compounds in the exponential growth phase was experimentally confirmed. The most pronounced effect of growth suppression was found in the presence of lactobacilli protoplasts.