A Comparative Analysis of Cytotoxicity of Three Different Root Canal Sealers

Aim: This study aims to evaluate Well-Root ST in comparison with CeraSeal and AH Plus regarding cytotoxicity using (MTT) assay on human epithelial type 2 (HEp-2) cells. Materials and Methods: Teflon mold was used to fabricate disc samples of each root canal sealer. Samples were divided into 3 groups: group (A) (Well-Root ST), group (B) (Ceraseal) and group (C) (AH Plus) in two evaluation periods (24h and 72h). All samples of root canal sealers were mixed according to the manufacturer’s instructions and the discs were allowed to set in a at 37°C for 24 hours before extraction. Extract collected at each time point was diluted to various concentrations of 12.5%, 25%, 50% and 100% with DMEM to create a total of four different concentrations of each extract. DMEM incubated for 24 hours served as the control. (HEp-2) cell line was seeded in 96 well micro-titer plates and cultures were then subjected to 100 μL of the (12.5%, 25%, 50% and 100%) extracts medium while cell cultures with supplemented DMEM were used as controls. The plates were left in the incubator for 24 or 72 h before the cytotoxicity evaluation was carried out by (MTT) assay. Viability percentage was calculated and cytotox