Throughout the world, significant areas of agricultural land are polluted with hydrocarbons. Their microbiological purification is hampered by the presence of residual amounts of pesticides (herbicides, in particular), which inhibit the vital activity of oil-destructing bacteria. In addition, along with crude oil, heavy metals and salts (mainly chlorides) can enter arable soils, which, in turn, have a negative effect on microorganisms. Therefore, for the bioremediation of such territories, hydrocarbon-oxidizing bacteria resistant to the presence of additional pollutants should be used. In the present work, three isolates were identified that are capable of active growth in a liquid medium with oil. Using time-of-flight matrix-activated laser desorption-ionization (MALDI-TOF) mass spectrometry of the cellular protein fraction, sequencing of the nucleotide sequence of the 16S rRNA gene and phylogenetic analysis, the species of microorganisms belonging to the genus Acinetobacter was determined. The bacteria had a significant ability to biodegrade oil (71.8-74.1%), which was estimated from the degree of destruction of the aliphatic oil fraction by the gas chromatographic method after p
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