Human Immunodeficiency Virus (HIV) is a deadly sex-transmitted infection (STI) that remains a worldwide public health concern. Despite the progress recorded in the HIV prevention, we still observe high HIV prevalence especially among Men who have sex with Men (MSM). Within this latter group, the prevalence of HIV is reported to be higher and can therefore be transposed into the general population through the bisexuals. In the Democratic Republic of Congo (DRC), the response to HIV/AIDS among MSM requires updated data on the magnitude of the infection, the sexual practices and behaviors which are key factors in the pandemic spread. This study aimed to determine the prevalence of HIV and to describe the sexual practices and behaviors among MSM recruited in Kinshasa, DRC. We conducted a prospective cross-sectional study from March 2014 to December 2017 to recruit MSM through the snowball technique in Kinshasa, DRC. Our data were collected on a paper-printed questionnaire via an interview followed by whole blood sample collection for ELISA assays. Of 323 MSM recruited, the MSM were single in 88.5% (286/323) and bisexuals in 61.7% (195/316). Nine percent of them were HIV positive (30/32
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