Modeling of liver hypoxia in an in vivo experiment

We studied an effect of the portal triad blood flow reduction on liver enzymes, the organ status and survival of laboratory mice. The study included Balb/c mice divided into groups (n=6 for each group): group 1 – controls, blood and liver were collected; group 2 – liver blood flow reduction for 20 minutes, blood and liver were collected 2 hours after the procedure; group 3 – liver blood flow reduction for 20 minutes, blood and liver were collected 24 hours after the procedure; group 4 – liver blood flow reduction for 20 minutes, and the follow-up during the next 14 days to assess the survival. The blood flow was reduced by occlusion of the portal triad: the animals underwent laparotomy, then the portal triad was isolated and a needle with suture material was brought under it; weights were attached to the ends of the suture material, and the vessels were occluded due to the tension of the ligature. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in both groups with reduced blood flow were statistically significantly higher than in the control group (p<0.05), and also statistically significantly higher in the group with blood sampling 24 hours aft