Introduction: Urinary tract infection (UTI) is one of the most common bacterial infections of childhood. Among febrile infants, sick children and older children with urinary symptoms, 6%–8% will have a UTI. Prevalence varies with age, peaking in young infants, toddlers and older adolescents. Aims: To identify causative bacteria causing UTI in under five children and their antibiotic susceptibility pattern. Materials and methods: This descriptive cross-sectional study was conducted from April 2021 to October 2022 in the Department of Microbiology, Bankura Sammilani Medical College, Bankura. A total of 271 under-five children with urinary symptoms were included, representing all urine samples received from this age group during the study period. Result: 99 (36.90%) patients were affected in Escherechia coli., 35 (12.91%) patients were affected in Klebsella pnumoniae, 4 (1.40%) patients were affected in Citrobactor koseri, 42 (15.49%) patients were affected in Klebsella oxytoca, 11 (4.05%) patients were affected in Enterobactor Species, 24 (8.85%) patients were affected in Staphylococcus aureus, 7 (2.58%) patients were affected in Pseudomonas aeruginosa, 39 (14.39%) patients were affe
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