Introduction: Healthcare-associated infections (HCAIs) are more likely to occur in healthcare settings, driven by factors involving host, pathogen and environmental elements, including healthcare personnel. Infection prevention and control (IPC) practices are crucial to mitigate pathogen transmission, with hand hygiene being especially effective. Despite knowledge of guidelines, adherence remains inconsistent, necessitating structured IPC education Aim: To evaluate the impact of a structured, multifaceted educational intervention on the knowledge and compliance of healthcare workers regarding IPC practices at a tertiary care centre. Methodology:A tertiary care facility in central India served as the site of this observational study for over eight months (Feb–Sept 2024), involved 394 participants, including nursing staff, interns, postgraduate students and hygiene warriors.The intervention includedinteractive CMEs, hands-on training, educational videos, and participatory activities like role plays and competitions. Pre-and post-intervention assessments measured knowledge improvement, while post-intervention door-to-door evaluations identified practical compliance. Results:The i
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