Background. Distribution of root nodule bacteria of soybean in soils of Ukraine is the result of intensive cultivation of soybeans over the last 20 years. During the observation the structure of soybean rhizobia populations for the first time we have determined the strains which significantly differ in phenotypic properties from typical slow-growing bacteria of B. japonicum species previously described. These strains are characterized by high speed growth and we tentatively called them “stains with intensive growth”. The aim of our work was to investigate the genotypic properties of microsymbionts of soybeans with different rates of growth spreading in soils of Ukraine. Materials and methods. The 16S rRNA gene and intergenic 16S-23S rRNA region of six strains - typical representatives of soybean nodule bacteria with slow- and intensive growth-rates was carried out. The strains were picked up from different Ukrainian soils. Results. Analysis of the 16S rRNA nucleotide sequences showed the 100% similarity of slow-growing strains to B. japonicum USDA 6T and USDA 4 ones. This analysis proved propinquity of strains with intensive growth to the strain B. japonicum USDA 127 (USDA 123 grou
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