Seven (7) yeast strains were isolated from pineapple pulp and six (6) of them were tentatively identified as Saccharomyces species. Attempts to select prototrophs from these isolates considered eleven (11) different markers. Two (2) of the yeasts were prototrophic for tryptophan (trp-) and isoleucine/valine (iiv) marker respectively. Two (2) of the seven (7) yeasts isolated were self-sporulating. None of the isolates exhibited properties indicative of respiratory deficiency. This was also true for the hybrid produced. Two mating programmes were attempted involving the isolates and a standard Laboratory strain and between the isolates. Six (6) out of the twenty-one (21) attempts produced hybrids. All the hybrids sporulated.
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