Summary. A series of studies on enhancing the cytotoxic effect of spermine on human prostate cells by affecting the enzymes of its metabolism has been continued. Aim: to investigate effects of pargyline, spermine and their combinations the survival, ζ-potential and surface charge and cytomorphological properties of human prostate cancer cells in culture. Object and methods: studies were conducted on cell cultures: differentiated androgen-dependent LNCaP cell line and low-differentiated androgen-independent DU-145 cell line. Cell survival was determined in the trypan blue assay. The effect of the investigated compounds, both individually and in combination, on the viability and proliferative activity of cells was assessed by the colorimetric method – by staining living cells with a solution of crystal violet in methanol. Biophysical parameters (ζ-potential and surface electric charge density) were determined according to the method cellular microelectrophoresis. Cytomorphological characteristics were studied on preparations of cells grown on coverslips, fixed and stained with hematoxylin and eosin (magnification 1000×). Results: for LNCaP cells, the IC50 of pargyline was 2.2 mM, and
📖 افتح في inklap 🔗 DOI 📮 اطلب بحثاً