The deposited by us highly active bacteriocin producer Pseudomonas aeruginosa UСM B-333 synthesizes pyocins, which intensively inhibit phytopathogenic strains of Pseudomonas syringae — plant pathogens. This strain produces pyocins S1 and S5, as well as microcin-II-like bacteriocins. The aim of this work was to check the presence of other pyocins in P. aeruginosa UCM B-333. Methods. The concentrated bacterial lysate of P. aeruginosa UCM B-333 was separated by ion-exchange chromatography on DEAE cellulose. The fraction with studied bacteriocin was further purified by gel filtration on Sephadex G-75. To determine the belonging of investigated pyocin to a certain subtype, its molecular weight, antimicrobial activity, kinetics of the effect on sensitive microorganisms, and serological homology with carotovoricins of Pectobacterium carotovorum were studied as well as the ability to interact with siderophore receptors and nuclease activity were tested. Results. The isolated pyocin is a protein with a molecular weight of the active part of pyocin of 43.4 kDa and an immune protein — of 9 kDa. Th is substance is characterized by nonspecific DNase activity and affects sensitive cells by the s
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