Aim. The aim of the research was to determine changes in content and composition of Streptomyces massasporeus CNMN-Ac-06 strain biomass lipid complex during cultivation in various nutrient media after long-term storage. Methods. To obtain the inoculum, S. massasporeus CNMN-Ac-06 strain was cultivated in Dulaney medium. For biomass accumulation, inoculum was cultivated in M-I, SP-I and SP-III. The amount of biomass was determined on the 5th day of culture growth. Intracellular lipids were extracted from streptomycete biomass by Folch method modified in our laboratory. The qualitative and quantitative characteristic of lipid complex composition was determined by thin-layer chromatography. 10% solution of phosphomolybdic acid in ethanol was used as developer. The quantity of individual lipid fractions was determined by densitometry. Results. The studied strain was cultivated in three nutrient liquid media. Cultivation of the strain in M-I medium increases the biomass yield up to 11.53 g/l. In case of SP-III, on the contrary, at the beginning of research, the biomass yield was higher than after storage. The best result of the synthesis of total lipids was noted after SP-I medium use. A
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