Abstract Objective The aim of the study is to determine knowledge of nursing students about taking and transferring of bio-chemical laboratory samples. Methods The study is descriptive and was conducted during the spring semester of the 2016–2017 academic year and 118 volunteer final year nursing department students were included in the study. As a data collection tool, a questionnaire which was prepared by the researcher. Results Eleven percentage of the final year students of the nursing department answered the information that should be in the laboratory sample request document and 83.9% responded correctly to the optimal blood-letting time for the biochemical analysis. It was determined that 52.5% of the students correctly know whether the sitting, lying or on-foot blood-letting positions affect the blood values or not, and 45.8% know the reason why blood-letting is performed in sitting or lying positions. 57.1% of the students correctly identified the medium urine s
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