Background: Cadmium exposure affects the expression of the DMT1 gene and the function of its transporter protein, impacting the transport and accumulation. This study investigates genetic polymorphisms to understand better the pivotal role of genetic factors in cadmium-related diseases within environmental health research. Objective: The study sought to examine the intronic IVS4+44C/A polymorphism in the divalent metal transporter 1 (DMT1) gene among individuals aged 35-60 residing in regions contaminated with cadmium. Materials and methods: Blood samples were collected from 306 genetically unrelated individuals (158 females and 148 males). The DMT1 IVS4+44C/A polymorphism was determined using restriction fragment length polymorphism (RFLP) and Sanger sequencing methods. Urinary cadmium levels were measured with graphite furnace atomic absorption spectrometry (GFAAS). Statistical analyses included Hardy-Weinberg equilibrium testing, analysis of variance (ANOVA), and student’s t-tests. Results: The geometric mean of urinary cadmium levels were significantly higher in females (4.03±4.15 µg/gm creatinine) than in males (2.62±2.73 µg/gm creatinine). Remarkably, 85% of females and 66
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