Design and Research of Multi-outlet Microfluidic Chip Based on Pesticide Residue Detection

With excellent mixing efficiency, microfluidic chip technology has shown great development advantages and potential in pesticide residue detection. Aiming at the problems of limited sensitivity and insufficient integration automation degree of three-dimensional microfluidic chips in pesticide detection, a new type of microfluidic chip based on 3D printing technology was designed to improve pesticide detection accuracy, including 3 injection ports and 4 detection ports. The chip structure meets the expected goal of fully mixing test samples and detection reagents in laminar flow state. Three different deflector column structures and one control structure were designed, and the chip mixing performance was verified through simulation experiments and liquid mixing pre-experiments. The injection flow rates of 0.06 m/s, 0.09 m/s and 0.12 m/s for test samples and 0.12 m/s, 0.18 m/s and 0.24 m/s for detection reagents were set to detect the mixing effect of microfluidic chips under different conditions. Light curing 3D printing technology is used to print microfluidic chips based on materials. Through electron microscope observation, experimental test and simulation results, the chip print