Several prostatic tissue DNAs have been examined for their content of simian-sarcoma-virus-related sequences. This was done by hybridizing fragmented prostatic DNA with simian sarcoma virus complementary DNA (cDNA) synthesized in vitro. The tissue DNAs contained limited amounts of virus-related sequences. The content ranged from about 10 to 60 pg of viral DNA per milligram of cell DNA, depending on the tissue. This corresponds to approximately one viral genome equivalent per 20–100 cell genomes. A clear correlation between viral DNA content and histopathology of the tissue – normal, hyperplastic and neoplastic – has not emerged from the tissue DNAs examined thus far. The trend appeared to be for the hyperplastic tissue to contain more virus-related sequences than normal or neoplastic tissue. The melting point of hybrids of prostatic DNA and viral cDNA was 15°C lower than that of native prostatic DNA. This can be interpreted to suggest that prostatic DNAs contain a distribution of short segments of sequences that hybridize with viral cDNA.
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