The elongation factor Tu (EF‐Tu) isolated from Escherichia coli cells that have undergone the stringent response is predominantly complexed with guanosine 5′‐diphosphate 3′‐diphosphate. This complex can be separated by anion‐exchange chromatography from EF‐Tu · GDP‐ Unlike EF‐Tu complexed with guanosine 5′‐triphosphate 3′‐diphosphate, the complex with guanosine 5′‐diphosphate 3′‐diphosphate cannot form a ternary complex with aminoacyl‐tRNAs as can be shown directly by hydrolysis protection experiments.
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