The action of 9‐bromomethylanthracene on poly(A) either single stranded or complexed with poly(U) has been investigated. Reaction of the polycyclic hydrocarbon occurs specifically on the amino group of adenylic residues in the polymer whereas the N1 position is also conjugated in the monomer using the same experimental conditions. Poly(G) reacts to a lower extent than poly(A) with the formation of one conjugate instead of two for the monomer. Cross linking congujation occurs between the two strands of DNA. The different factors governing the specificity and the extent of the reaction of this reagent with polynucleotides are discussed, the different geometry of double‐stranded structure inducing various reactions of conjugation. The extent of reaction was tested for several polynucleotides and their complexes. The B form of DNA was found to be the most sensitive one. The conjugates provide convenient labels for conformational studies of polynucleotides using fluorescence spectra and polarisation data.
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