inklap

Expression and genetics of caprine haemoglobins

M. BRAEND, L. L. NESSE, G. D. EFREMOV · Animal Genetics · 1987

Summary. The expression of haemoglobin (Hb) has been studied in 260 Norwegian Dairy goats by the Immobiline technique at pH ranges 6.7–7.7, 6.9–7.6 and 6–9‐7.5. The majority of goats exhibited two‐ or four‐band patterns. In two‐band types the average ratio between the anodal and cathodal band was 74:26. PAGE with 8m urea distinguished three phenotypes for the beta chains, proving that the Hb variation described is in the beta chain. Segregation data in 106 complete sire‐dam‐offspring families agreed with the existence of four β globin alleles – A2, A4, A6 and A8. Twenty‐seven animals had reversed ratios (R) of Hb bands. In two‐band phenotypes the average ratio was 36:64. In 15 complete families where one of the parents had reversed ratio, eight offspring received the R type, indicating a simple genetic control. After urea PAGE the R animals all showed the same alpha chain phenotype which differed from that of goats having common ratios of bands. An additional polymorphism appeared in nine animals as three‐ and five‐band patterns which is assumed to be the result of heterozygosity for IIα and for IIα and β globin genes respectively.

📖 افتح في inklap 🔗 DOI 📮 اطلب بحثاً