AbstractIn foregoing work, we identified at least 5 distinct epitopes on human type II collagen (CII), using 8 murine monoclonal antibodies (mAb) against human CII, and suggested that a species‐nonspecific epitope on CII recognized by anti‐CII mAb termed 1‐5 is an arthritogenic epitope. We also found that antibody response against a selected epitope of human CII could be induced by immunization with rabbit anti‐idiotypic (Id) antibody against anti‐CII mAb. The author developed and characterized monoclonal anti‐Id antibodies against 1‐5 mAb recognizing a putative arthritogenic epitope. The author also investigated whether the anti‐Id mAb could regulate antibody response directed against a selected epitope recognized by 1‐5 mAb, and the induction of collagen‐induced arthritis in DBA/1J mice. DBA/1J mice intravenously preinjected with anti‐Id mAb to 1‐5, did not produce anti‐CII antibody expressing 1‐5 Id upon immunization with human CII. Furthermore, as the development of collagen‐induced arthritis (CIA) in DBA/1J mice pretreated with anti‐Id mAb to 1‐5 was significantly suppressed, anti‐Id mAb will be a useful tool for studying the regulation of antibody response to a selected epito
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