Summary The impact of environmental stimuli on the production of the widespread cyanobacterial hepatotoxin microcystin ( MC ) is under debate. Whereas transcriptional studies of the biosynthetic genes suggest a clear influence of light conditions on toxin production the data for the metabolite itself are inconsistent and highly strain‐specific. Here, we have reassessed the MC content by using two immunological detection techniques that allow a parallel quantification of MC in the methanolic extracts and the residual pellet fraction that contains high molecular weight proteins. Our results show a significant proportion of MC in the protein bound fraction in strains of M icrocystis and P lanktothrix and of the related toxin nodularin ( NOD )
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