Abstract The examples which have been given above in the cyanocobalamin and folic acid field illustrate the usefulness of the microbiologic assay of vitamins in investigate work. The application of these methods to routine determinations will follow, once the indications for their usefulness are established. Table 7 summarizes the vitamins for which we have developed assay methods, the microorganisms used, and their sensitivity. The methods described are much simpler than they may appear to be at first sight, and they require no expensive or special equipment. They can become in due course routine procedures and will contribute to one of the lesser known chapters of clinical chemistry.
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