Abstract Introduction There is a risk of glass contamination with the use of single-dose glass ampoules. Complications of injection include infection and granuloma formation and this is widely described in anesthetic literature. To date, there is no data on the effect of different ampoule opening methods on the degree of glass contamination. Purpose This article explores different ampoule opening methods and determines if any method is superior to the others with respect to glass contamination frequency. This article also increases awareness of glass contamination and its potential complications in the radiology community. Methods A controlled trial was undertaken with 15 glass ampoules filled with normal saline, divided into three groups. The ampoules in each of the group were opened via each method: freehand, ampoule breaker, and ampoule opener. The solution was aspirated with an 18-gauge drawing-up needle, which was centrifuged and decantered to be placed onto slides and inspected under light microscopy to assess the glass contaminants. Results Between each cohort, the freehand opening provided the least number of glass particles with 42,
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