1. Assemblages of epilithic diatoms, macrophytes, benthic macroinvertebrates and fish were monitored over a 5‐year period (1988–92) in ten lakes and nine streams in the U.K., as part of the U.K. Acid Waters Monitoring Network. All organisms were categorized according to their functional or morphological characteristics and integrated to describe the food web at each site. In general, the flora and fauna of all sites were typical of oligotrophic, softwater systems subject to various degrees of acidification.2. Salmonids were the only fish caught at any site and brown trout were the dominant species. With only 5 years of data it was not possible to test for directional changes in fish populations at each site. Among sites, fish density was positively associated with pH, and this did not vary between lake outflows and streams or between fish of different age class (0 +, 1 +). Condition factor, reflecting fish health, was not associated with pH among sites, but was negatively associated with fish density and, on average, was higher for fish in streams than those in lake outflows.3. Variability in the diatom, macrophyte and invertebrate data sets were quantified in three ways using mul
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