The IL‐2 mediated amplification of cellular cytotoxicity

AbstractHigh dose [> 1 nM or 30 IU] interleukin‐2 (IL‐2) can induce MHC ‐unrestricted killing from various lymphoid populations. Although it is well established that CD16+ NK cells are the major source of blood‐derived LAK precursors, other lymphoid cells, including several CD3+ T subsets can be a source of precursor activity. We hypothesize that most, if not all, lymphocytes with cytolytic potential may eventually express MHC‐unrestricted killing, when provided with adequate IL‐2 to intiate required secondary cytokine production. This perspective article presents our cumulative data supporting the role of secondary cytokines in the IL‐2 initiated activation of MHC‐unrestricted killing, first by our observations of synergy with the exogenously added TNFs or IL‐1s in combination with low dose IL‐2, and then by the evidence of endogenous cytokine production and response in lymphocytes stimulated with high dose IL‐2.Understanding the amplification mechanism(s) of the various effector arms of the immune system is critical to the eventual regulation of graft rejection, autoimmune phenomena, and potentially to the treatment of cancer. Our studies have focused on the cytotoxic lymphocy