AbstractKinetic analysis has provided a detailed quantitative description of the mechanism of actin polymerization as well as the methods to analyze the mechanisms of action of actin‐binding proteins. In Acanthamoeba, five different proteins regulate the pool of monomers available for polymerization, cap the end of filaments, sever filaments, and cross‐link filaments. Remarkably, many of these interactions involve very‐low‐affinity bonds between the protein molecules.
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