A 2‐photon microscope image of the dura in a living mouse, taken through the thinned skull (blue background). The mouse is engineered to express green fluorescent protein in monocytic cells including macrophages, and was injected intravenously with a red dye to label the blood vessels. The three dimensional relationships of dural and pial macrophages as well as dendritic cells and their activation by cortical spreading depression is explored in Schain et al., pages 508–521. Ann Neurol 2018;83:1–1
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