Abstract Recipes Acid, concentrated stock solutions Acid precipitation solution Alsever's solution Ammonium hydroxide, concentrated stock solution Ammonium acetate, 10 M Ammonium sulfate, saturated ATP, 100 mM Base, concentrated stock solutions BBS (BES‐buffered solution), 2× BCIP, 5% (w/v) BSA (bovine serum albumin), 10% (w/v) CaCl2, 1 M Carbonate buffer Denhardt solution, 100× DEPC (diethylpyrocarbonate)‐treated solutions DNase I, RNase‐free (1 mg/ml) dNTPs: dATP, dTTP, dCTP, and dGTP DPBS (Dulbecco's phosphate‐buffered saline) DTT (dithiothreitol), 1 M EDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0) Ethidium bromide staining solution FBS (fetal bovine serum) FBS, heat inactivated Formamide loading buffer, 2× HBSS (Hanks' balanced salt solution) HCl, 1 M HeBS (HEPES‐buffered saline) solution, 2× KCl, 1 M 2‐ME (2‐mercaptoethanol), 50 mM MgCl2, 1 M MgSO4, 1 M MOPS buffer NaCl, 5 M NaOH, 10 M PCR amplification buffer, 10× Phenol, buffered Phenol/chloroform/isoamyl alcohol, 25:24:1 (v/v/v) Phosphate‐buffered saline (PBS) Phosphate‐buffered saline containing potassium (KPBS) PMSF (phenylmethylsulfonyl fluoride), 100 mM Potassium acetate buffer, 0.1 M Potassium phosphate buffer
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