Isolation and Maintenance of <i>Brachyspira</i> Species

AbstractBrachyspira species are anaerobic spirochetes inhabiting intestinal tracts of animals and humans. Several species cause transmissible intestinal diseases of swine and birds. This unit describes methods for the isolation of Brachyspira from fecal samples, cultivation on liquid and solid media, and long term and short term preservation of Brachyspira species. Curr. Protoc. Microbiol. 22:12D.1.1‐12D.1.14. © 2011 by John Wiley &amp; Sons, Inc.