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Laboratory Maintenance of <i>Borrelia burgdorferi</i>

Wolfram R. Zückert · Current Protocols in Microbiology · 2007

AbstractThis unit describes the propagation of Borrelia burgdorferi as well as other “cultivable” Borrelia species in Barbour‐Stoenner‐Kelly‐II (BSK‐II) medium. It supplies a detailed recipe for BSK‐II, as well as protocols for standard liquid culture, the generation of frozen Borrelia stocks, and the plating of B. burgdorferi cells in solid BSK‐II medium.

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