AbstractMycobacterium abscessus, is an emerging, rapidly growing pathogen, with the ability to cause chronic lung disease. This unit covers background information and laboratory maintenance procedures for this bacterium, including growth in liquid and on solid medium. It also contains recommendations concerning long‐term strain storage. M. abscessus is a Biosafety Level 2 organism, and the required safety measures are also discussed. Curr. Protoc. Microbiol. 18:10D.1.1‐10D.1.12. © 2010 by John Wiley & Sons, Inc.
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