AbstractThis unit describes general procedures for the lab cultivation and storage of the Gram‐positive facultative intracellular bacterium Listeria monocytogenes. The basic protocols are relevant for a wide scope of applications including microbial genetics and both in vitro and in vivo infection studies. Commonly used L. monocytogenes strains, serotypes, and growth parameters are also discussed. Curr. Protoc. Microbiol. 31:9B.2.1‐9B.2.7. ©2013 by John Wiley & Sons, Inc.
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