Growth and Laboratory Maintenance of <i>Pseudomonas aeruginosa</i>

AbstractPseudomonas aeruginosa is a common, free‐living, Gram‐negative bacterium that can cause significant disease as an opportunistic pathogen. Rapid growth, facile genetics, and a large suite of virulence‐related phenotypes make P. aeruginosa a common model organism to study Gram‐negative opportunistic pathogens and basic microbiology. This unit describes the basic laboratory growth and maintenance of P. aeruginosa. Curr. Protoc. Microbiol. 25:6E.1.1‐6E.1.8. © 2012 by John Wiley &amp; Sons, Inc.