AbstractThe causative agent of the respiratory disease whooping cough, Bordetella pertussis, is a nutritionally fastidious microorganism but can be grown with relative ease in research laboratories. Stainer‐Scholte synthetic broth medium and Bordet‐Gengou blood agar both support growth of B. pertussis and are commonly used. B. pertussis prefers aerobic conditions and a temperature range of 35° to 37°C. Appropriate laboratory safety protocols are required to prevent the generation of aerosols, which could potentially spread this highly infectious agent. Curr. Protoc. Microbiol. 15:4B.1.1‐4B.1.9. © 2009 by John Wiley & Sons, Inc.
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