Production of High‐Titer Lentiviral Vectors

AbstractConventional retroviral vectors are useful for gene delivery because of their large cloning capacity and ability to integrate into the chromosome of the target cell. However, their inability to transduce nondividing cells limits their utility. In contrast, lentiviruses, a retrovirus subfamily, can infect both non‐replicating and dividing cells. This unit describes the production of human immunodeficiency virus‐derived vectors by transient transfection of 293T cells. In addition, the multiple safeguards engineered into the latest version of HIV‐based vectors are described.Conventional retroviral vectors are useful for gene delivery because of their large cloning capacity and ability to integrate.