Immunoaffinity Chromatography

AbstractImmunoaffinity purification is a powerful technique for isolating proteins. Purifications of 10,000‐fold or more can often be achieved in one step. This unit describes the isolation of soluble or membrane‐bound protein antigens from cells or homogenized tissue by immunoaffinity chromatography. The technique involves the elution of a single protein from an immunoaffinity column after prior elution of nonspecifically adsorbed proteins. An alternate protocol describes the use of batch purification of antigens which can shorten the column loading time.