Abstract Recipes Acid, concentrated stock solutions Ammonium hydroxide, concentrated stock solution Artificial cerebrospinal fluid (aCSF) Calcium‐ and magnesium‐free Hank's buffered salt solution (CMF‐HBSS) Denhardt's solution, 100× Diethylpyrocarbonate (DEPC) treatment of solutions DNase I, RNase‐free (1 mg/ml) Dulbecco's modified Eagle medium (DMEM), supplemented EDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0) Ethidium bromide staining solution Fetal bovine serum (FBS) Gel loading buffer, 6× HBSS (Hanks' balanced salt solution) LB medium (Luria broth) and LB plates PBS (phosphate‐buffered saline) Phenol, buffered Phenol/chloroform/isoamyl alcohol, 25:24:1 (v/v/v) Polylysine‐coated tissue culture surfaces Potassium phosphate buffer, 0.1 M RNase a stock solution, DNase‐free, 2 mg/ml SDS sample buffer (for discontinuous systems) Sodium acetate, 3 M Sodium phosphate buffer, 0.1 M SSC (sodium chloride/sodium citrate), 20× SSPE (sodium chloride/sodium phosphate/EDTA), 20× TBE (Tris/borate/EDTA) electrophoresis buffer, 10× TE (Tris/EDTA) buffer Tris‐buffered saline (TBS) Tris⋅Cl, 1 M
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