Overview of Gene Targeting by Homologous Recombination

AbstractOverview of Gene Targeting by Homologous Recombination (Richard Mortensen, University of Michigan Medical School, Ann Arbor, Michigan).This overview describes the basic methodology and applications of homologous recombination. Both insertion and replacement constructs are discussed along with methods of selection. Gene mutation and inactivation to produce a knockout animal can be accomplished in several ways, and the Cre‐loxP system is described for this purpose in some detail.