Mutagenesis Approaches for Elucidation of Protein Structure‐Function Relationships

AbstractSeveral mutagenesis strategies have been used to allow the identification of specific receptor‐ligand interactions to support the docking of ligands within various receptor models. The strengths and limitations of each strategy are discussed in this overview. Large‐scale mapping is described using deletion and chimeric receptors. Fine tuning with single residue replacements is also covered along with two‐dimensional approaches.