Fluorescence Recovery After Photobleaching (FRAP) with a Focus on F‐actin

AbstractIn this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein‐protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area. The data gained in FRAP studies is qualitative and yields insight into relative binding affinity, binding characteristics, and the effect of treatments or mutations on protein mobility. Curr. Protoc. Neurosci. 61:2.17.1‐2.17.12. © 2012 by John Wiley & Sons, Inc.