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Loading Neurons with Dextran‐Conjugated Calcium Indicators in Intact Nervous Tissue

Kerry R. Delaney · Current Protocols in Neuroscience · 2010

AbstractDextran‐conjugated Ca2+ indicators are retained well in neurons for many days following loading in intact or semi‐intact brain tissue. Methods for loading neurons, as well as discussion of the unique properties of dextran‐conjugated dyes which need to be considered for their use, are presented. Curr. Protoc. Neurosci. 50:2.5.1‐2.5.11. © 2010 by John Wiley & Sons, Inc.

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